Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Cell viability and antibiofilm potential of Mo -CBP3-PepI against K. pneumoniae .

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Inhibition

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Mechanism of action of Mo -CBP3-PepI assessed by fluorescence microscopy. In all experiments, Mo -CBP3-PepI was used at a concentration of 31.26 μg mL −1 . ( A , B ) control K. pneumoniae cells for PI uptake assay. ( E , F ) Mo -CBP3-PepI-treated K. pneumoniae cells for PI uptake assay. Red fluorescence ( F ) indicates pore formation induced by Mo -CBP3-PepI. ( C , D ) control K. pneumoniae cells for FITC-Dextran uptake assay. ( G , H ) Mo -CBP3-PepI-treated K. pneumoniae cells for FITC-Dextran uptake assay. Green fluorescence ( H ) indicates 10-kDa -sized pore formation induced by Mo -CBP3-PepI. ( I , J ) control K. pneumoniae cells for ROS overproduction assay. ( K , L ) Mo -CBP3-PepI-treated K. pneumoniae cells for ROS overproduction assay. Green fluorescence ( L ) indicates pore formation induced by Mo -CBP3-PepI. Bars indicate 100 μm.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Fluorescence, Microscopy, Concentration Assay, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Scanning electron microscope of K. pneumoniae cells treated with Mo -CBP3-PepI. ( A ) control K. pneumonia cells treated with DMSO-NaCl solution. ( B – H ) K. pneumoniae cells treated with Mo -CBP3-PepI 31.26 μg mL −1 .

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Microscopy, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Distribution of K. pneumoniae proteins obtained after treatment with Mo -CBP3-PepI. ( A ) Venn diagram shows the dispersion of total proteins of control and Mo -CBP3-PepI-treated cells. ( B ) Represents the fold-change value of overlapping proteins, which were found in both groups. The vertical bar indicates the color scale according to each protein’s fold-change value.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Dispersion, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Classification proteins from K. pneumoniae cells identified by LC-ESI-MS/MS analysis. The overlapping proteins (found in both control and treated) were classified based on biological process and molecular function.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Tandem Mass Spectroscopy, Control

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Differentially accumulated proteins identified by ESI-LC-MS/MS.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Clinical Proteomics, Membrane, Modification, High Molecular Weight

Journal: Antibiotics

Article Title: New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo -CBP 3 -PepI against Klebsiella pneumoniae

doi: 10.3390/antibiotics11121753

Figure Lengend Snippet: Scheme showing the mechanisms of antibacterial action of Mo -CBP3-PepI against K. pneumoniae . This scheme was produced only with proteins that decreased in abundance. The numbers explain important process. All them are explained in the text.

Article Snippet: Cytadherence high molecular weight protein 2 , P75471 , Mycoplasma pneumoniae (strain ATCC 29342/M129) , Cytoplasm , 1.682.

Techniques: Produced

Journal:

Article Title: Detection of Macrolide Resistance in Mycoplasma pneumoniae by Real-Time PCR and High-Resolution Melt Analysis ▿

doi: 10.1128/AAC.00582-08

Figure Lengend Snippet: Macrolide-resistant clinical isolates and MIC data

Article Snippet: Reference strains M129 (ATCC 29342D) and FH (ATCC 15531) were tested along with 100 clinical isolates submitted to or isolated within the Respiratory Disease Branch of the CDC for testing between 1991 and 2008.

Techniques: Isolation, Mutagenesis